Abstract: Environmental education was accepted in the 1960s as a solution to public pollution and natural environment destruction problems in Japan. After 60 years of development, environmental education has covered biodiversity and sustainable development. Taking Japan’s Yambaru National Park as an example, this paper explains the environmental education and natural experience activities of national parks from the aspects of policy system, eco-tourism and biodiversity conservation. It concludes that Yambaru National Park has carried out eco-tourism-based environmental education, centering on resident groups and enterprises, with support and guidance of local governments and protection centres. Led by the Yambaru Wildlife Protection Center directly under the Ministry of Environment of Japan, it has fulfilled biological protection such as expelling alien species and protecting endangered animals and plants, and environmental education based on biodiversity for local residents, students and tourists.