Abstract: The spatial structure characteristics of landscape are closely related to residents’ behavior characteristics and space use efficiency. Aiming at the two structural elements of garden road system and node system, this paper first makes quantitative analysis of the landscape spatial structure from three aspects, using the spatial syntax, ArcGIS core density analysis and UNA tools: integration degree of garden road, node density and space-time selectivity. Secondly, through a series of data statistics, it carries out matching analysis of the spatial structure and behavior of the original design scheme, exploring whether there is a good correlation between the structural design of landscape and the temporal and spatial distribution of visitors. Thirdly, combined with the practical scheme, it corresponds the calculation results to the scheme design drawing, identifying the regions with low matching degree. It has thus formed the research ideas and methods from spatial structure prediction of human flow distribution to matching degree analysis, and then feedbacking the analysis results to design. It provides a reference for the optimization and adjustment of targeted schemes.