Abstract: Ethnic cultural ecology reserve is an experimental and innovative aspect of China’s overall protection of ethnic intangible cultural heritage. The key problems is how to reasonably determine the protection patterns and delineate the core protection areas, cultural routes and cultural nodes according to the quantity level, distribution density and transmission path of intangible cultural heritage. Taking the town as a unit, this research applies GIS to establish a spatial database, builds the value evaluation model of three baseline layers and twelve index layers, determines the weight, obtains the comprehensive value, puts the value score into the spatial interpolation for analysis, and clusters the analysis results. This paper analyses the inheritance and dissemination paths of intangible cultural heritage, determines cultural routes, obtains protection patterns by means of superposition, constructs the technical methods, and applies the technical methods to the planning practice of Southeast of Guizhou Province ethnic cultural ecology reserve.