Abstract: China is currently in a transitioning stage from rapid urbanization to higher quality development. However, due to multiple stresses of global warming, urban heat island and aging population, the urban climate problem will become increasingly severe in the future. Urban ventilation path is an effective urban climate mitigation strategy. It is hard to fully understand and scientifically describe the characteristics of wind environment precisely by a single model or scale due to its complexity and heterogeneity. Taking the core zones of Dalian, a peninsula city in North China as an example, an interdisciplinary research was conducted by applying meteorological observation, remote sensing, mesoscale climate simulation, urban form indices and geospatial analysis synthetically. A quantitative and precise excavation study of ventilation paths was made based on a comprehensive description of urban topography, thermal and wind environment, land use and urban morphologies. Accordingly, the urban planning, management and control strategies, and design guidelines were formulated for ventilation paths considering the spatial planning, width, land use types and landscape design. This research provides valuable reference for building a more ecological friendly livable city.