Abstract: The evolution of landscape patterns inevitably affects the supply of ecosystem services. It is of great significance to explore the relationship between them for regional sustainable development. Based on the Landsat images of Lijiang River Basin in the 1986, 2001, 2016 period, this research extracts the landscape types to analyze the impacts on ecosystem services values of landscape patterns. The results show that woodland has been the dominant landscape of the Lijiang River Basin for 30 years, with an increase in areas. The areas of farmland and waterbody decreased, construction land and bare land increased, and grassland first decreased and then increased. The ecosystem services value constantly increased, with a net value of 750 million yuan. Woodland contributed the most to the ecosystem services value, serving as the main source of the increase. The function of ecosystem regulation and support was greater than that of production and culture. There were obvious spatial differences in the ecosystem services value, low in the middle valley area and high in the surrounding mountains. The expansion of the dominant patch areas and good natural connectivity between patches, and the decrease of uniformity and heterogeneity of landscape types were conducive to the increase of the ecosystem services value. The research reveals the relationship between landscape patterns and ecological services changes, which provides scientific references for landscape sustainable management in Lijiang River Basin.