Abstract: The in-depth study of the spatial morphological differences of urban heat islands is of great significance to understand urban heat island effects and improve the urban microclimate. Taking the downtown area of Guangzhou as an example, this research identifies the spatial patterns of heat islands with the spatial types of the regional circles, and extracts typical heat island blocks based on the LCZ theory for data analysis. It summarizes the spatial morphological characteristics from the aspects of land use type, surface coverage and construction intensity. The results show that the study area presents a wavy heat island spatial pattern of “double heights inside and outside” from the center to the periphery. It extracts four typical heat island blocks, namely, high-density residences, industries, high-density services, and facility agriculture. The polarization of building density, high proportion of industrial land, high proportion of impervious surface and low vegetation coverage are the main reasons for the strong heat islands in the study area. Based on this, the research puts forward an operational morphological differentiation control strategy, which enriches the research results on the spatial morphology of urban heat islands, providing a reference for the urban ecological planning and construction in hot and humid regions of the country.