Abstract: Current strategies for biodiversity conservation largely aim at protecting remnant natural areas in urban built environment or specific species, while paying little attention to biodiversity conservation integrated with urban design strategies. In the concept of wildlife-inclusive urban design, the demand of animals and humans are incorporated in the process of design strategies, serving as creative paths for the conservation of biodiversity from the perspective of spatial planning and design. The method of literature analysis was used to systematically review and discuss the key concept, design strategies and current applications of wildlife-inclusive urban design, and explored the symbiosis strategy regarding the coexistence of target species survival and urban development. In addition, considering the actual demand of biodiversity conservation and territory spatial planning, the application path of wildlife-inclusive urban design was discussed, and thus proposed three key points of application path including merging of planning control, participation of comprehensive expert team and multiple interest groups. Results of this study provided new ideas and methods for biodiversity conservation in urban human settlement environment, so as to facilitate the development of the innovative concept of wildlife-inclusive urban design in the aspects of theoretical framework and empirical application.