Abstract: Singapore has embarked on a greening programme since 1963, evolving from a garden city to a biophilic city in a garden. To effect biodiversity conservation in Singapore, the National Parks Board formulated a Nature Conservation Masterplan and its implementation with a focus on the use technological innovation is highlighted in this paper. The Singapore index on cities’ biodiversity which is a self-assessment tool for the evaluation of biodiversity efforts has been applied in cities globally.